We are looking to recruit an outstanding early career researcher who wishes to develop their own independent research program in the lab. While our lab is multidisciplinary with respect to experimental systems, the candidate will be expected to develop a project focused on the study of protein complexes essential for transcriptional regulation. Monash University and the EMBL-Australia program provide excellent infrastructure and resources. Outstanding applicants will be offered a supreme research environment and a competitive postdoctoral fellowship. The position will best suit self-driven individuals who, in the long run, wish to pursue a career as independent researchers. We are interested in candidates with a background in either structural biology or molecular and cell biology:
Postdoc position #1: Molecular and Cell Biology
The project: the successful candidate will lead a project aiming to determine how histone modifiers maintain genes in an off-state during development — a process often referred to as 'epigenetic repression'. Specifically, the project will aim to understand how the robustness of epigenetic repression is achieved at the molecular level, to what extent it is essential in order to maintain cell identity and how it is dysregulated in disease.
Environment and resources: the successful candidate will have funding and access to utilise and develop cutting-edge technology, including single-cell RNA sequencing, forward genetic screens (including CRISPR screens), mass spectrometry-based proteomics, electron cryotomography and genome editing combined with functional assays and standard genomic applications as ChIP-seq, HiC, ATAC-seq and RNA-seq. The successful candidate will have access to world-class research platforms at Monash University and High-Performance Computing (HPC) server for data analysis. This study will complement other ongoing studies in the lab — led by other scientists — including structure determination of macromolecular complexes of epigenetic modifiers and chromatin using high-resolution cryo-EM and electron cryotomography, and quantitative biophysical and biochemical functional assays.
The candidate: we are looking for a highly motivated cell or molecular biologist with an outstanding publication track record in studying transcription regulation and who is capable and willing to develop his/her own research program within the lab. Required experience:
Hands-on experience with methods to study transcription and/or epigenetic regulation using mammalian cell cultures, including the implementation of next-generation sequencing techniques. Ideally, these would include some of the following methods: RT-qPCR, RNA-seq, ChIP-qPCR, ChIP-seq, iCLIP/PAR-CLIP, RIP, IP, IP with MS.
Hands-on experience in the generation of constructs and stable cell lines for the study of transcription regulation or epigenetics in cells. Ideally, these would include the utilization of genome-editing approaches using CRISPR-Cas9 for a knock-out, knock-in and rescue experiments.
Experience with forward genetic screens in mammalian cells.
Experience in imaging mammalian cells using either cryo-electron tomography, immunofluorescence microscopy or live-cell imaging.
Postdoc position #2: Structural Biology
We are looking for a highly motivated scientist that is fully independent and have an excellent track record in order to study the structural basis for epigenetic repression. We are developing methods for the reconstruction and for a structure-function study of chromatin and large epigenetic modifier complexes in vitro. We have on-site access to a state-of-the-art cryo-EM facility at Monash University (including Titan Krios and Talos Arctica electron microscopes), advanced mass spectroscopy facility, high-performance computing (HPC) clusters and we are located just across the road from the Australian Synchrotron: a walking distance from world-class beamlines for X-ray crystallography and SAXS.
Required experience in structural biology:
Extensive experience with structural biology applications of X-ray crystallography and/or cryo-EM (either single particle or tomography).
Extensive experience with the development of methods for the expression and purification of large multi-subunit macromolecule complexes for structure determination.
Experience working with complexes of proteins with cofactors as RNA, DNA and/or nucleosomes, including structure-function study in vitro.
Experience with complementing approaches for structural study, as SAXS or cross-linking mass spectrometry (XL-MS).